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injection of 50 mg/kg sodium pentobarbital (Sigma), and thoracic aorta was carefully dissected and maintained at 37 C in Krebs solution containing 118.2 mM NaCl, 4.7 mM KCl, 1.2 mM MgSO 4 , 25 mM NaHCO 3 , 1.2 mM KH 2 PO 4 , 1.9 mM CaCl 2 and 11.7 mM dextrose (pH 7.4, 95% O 2 , 5% CO 2 )

Even when a small fraction does reach the viable epidermis, the molecule has a short functional half-life unless it is stabilized in the formulation (encapsulation, copper chelation, or both) 1

By 4 months, 18.7% retained benefits compared to 0% in placebo recipients